Wondfo HIV Self-Test adopts double antigen sandwich immunochromatography method. Once the specimen and the buffer are added into the respective wells, they will migrate along the device by capillary action. The HIV-1 and/or HIV-2 antibodies bind to the colloidal gold-HIV antigen (gp36/41), and the complex is then captured by the HIV recombinant antigen (gp41 and gp36) immobilized in the test region (T). When the levels of HIV-1 or HIV-2 antibodies are at or above the limit of detection (LOD) of the assay, it will produce a visible colored band in the test region (T) and indicates a positive result. When the levels of HIV-1 or HIV-2 antibodies are zero or below the LOD, there is no visible colored band in the test region (T) indicating a negative result.
To serve as an internal procedure control, a colored line will appear at the control region (C).