General description:

The MagMAX Viral/Pathogen II (MVP II) Nucleic Acid Isolation Kit is
specifically designed to recover viral RNA and DNA from human
nasopharyngeal swabs. The kit utilizes MagMAX magnetic-bead technology
to isolate viral nucleic acid from the sample. This material should be
procured with S0001772 TaqPathCOVID-19 CE-IVD RT-PCRKit,1000RXN.

Manufacturer product reference: Thermo Fisher Scientific Inc.,
A48383

Technical Specifications:

Principle: Magnetic bead technology nucleic acid purification

Purification Target: Viral DNA/RNA.

Sample Type: Nasopharyngeal swabs.

Both Manual and Automated Workflow protocols

The Automated workflow protocols is designed for the KingFisher Flex
purification system (S0003917).

Fast procedure allows for 96 samples to be processed in <30 minutes.
Flexible protocol accommodates sample volume inputs from 200 to 400 µL
of viral transport medium.

No need for carrier RNA.

Elution volume of 50 µL.

Item Supplied with:

• 1x 550 mL Binding Solution.

• 1x 1,000 mL Wash Solution.

• 1x 20 mL Binding Beads.

• 1x 10 mL Proteinase K.

• 1x 100 mL Elution Buffer.

Item Required but not supplied:

A.For Manual workflow protocol

·Magnetic stand-96 (see IFU)

·Microplate shaker (see IFU)

·Incubator capable of reaching 65oC with slated shelves (see IFU)

·King Fisher Deep-well 96 plate

·Standard Optical 96-well plate for the eluate (see IFU)

·Vortex Mixer

·Clear Adhesive film

·Pipettes (Single and Multichannel 1.00 μL to 1,000.0 μL)

B.For Automated workflow protocol

·KingFisher Flex purification system (S0003917).

·KingFisher™ Flex 96 Deep-Well Heating Block (see IFU)

·King Fisher Deep-well 96 plate

·96-well plate for the tip comb (see IFU)

·KingFisher™ 96 tip comb for DW magnets (see IFU)

Other items required but not supplied

·Molecular Biology grade Absolute Ethanol

·Nuclease free water

·Extraction control (optional)

·Sterile conical tubes for reagent preparation

·Aerosol barrier pipette tips

·Microcentrifuge

Storage Transportation Condition:

Shipping condition (Room Temperature) Store at room temperature (15°C to
25°C)

Packaging & labelling:

Primary packaging: All contents of MagMAX™ Viral/Pathogen II Nucleic
Acid.

Isolation Kit

Secondary packaging: One Kit MagMAX™ Viral/Pathogen II Nucleic Acid.